Castle Lake is located in the Shasta National Forest of northern California, about 8 miles southwest of Mt. Shasta City (41.13' N, 122.22' W).
- I-5 northbound, travel to Mt. Shasta City and take the Central Mt. Shasta City exit. At the end of the off-ramp, turn left back over the freeway.
From I-5 southbound, drive to Mt. Shasta City and take the Central Mt. Shasta exit. Turn right.
- At the stop sign, turn left on Old Stage Road. The fish hatchery should be straight ahead of you.
- Keep to the right at the first fork.
- Continue passing golf course on left and afterward Lake Siskiyou on right.
- After crossing the dam, take a sharp left turn. There is a sign beforehand pointing to Castle Lake, approximately 7 miles up the road.
- Once you arrive at the Castle Lake parking lot, look for a trail near the bathrooms leading around the right side (north side) of the lake.
The lab is located about 0.25 mile down the trail. You can't miss it.
If you cross the outflow of the lake, you are going in the opposite direction.